New to RNA seq.. Need help!!!

You are currently viewing the SEQanswers forums as a guest, which limits your access. Click here to register now, and join the discussion

zizi

Junior Member

Join Date: Feb 2019

Posts: 1
- Share
- Tweet
#1

New to RNA seq.. Need help!!!

02-14-2019, 08:22 PM

Hello All

I am doing RNA seq using KAPA Stranded RNA-Seq Kit with RiboErase. I am following all the recommendations of the protocol but I seem to lose all my RNA during rRNA depletion step

Please suggest what to do.
Tags: None
luc

Senior Member

Join Date: Dec 2010

Posts: 469
- Share
- Tweet
#2

02-14-2019, 11:14 PM

How do you know? I would suggest to follow the library prep protocol all the way to the end and then assess the library or lack thereof.
Comment

Previous template Next

Topics	Statistics	Last Post
A New Method Makes Hantavirus Genome Analysis Faster and More Accessible by SEQadmin2 Started by SEQadmin2, 06-05-2026, 10:09 AM	0 responses 14 views 0 reactions	Last Post by SEQadmin2 06-05-2026, 10:09 AM
A New Single-Cell Method Maps DNA-Protein Interactions by SEQadmin2 Started by SEQadmin2, 06-04-2026, 08:59 AM	0 responses 24 views 0 reactions	Last Post by SEQadmin2 06-04-2026, 08:59 AM
Long-Read RNA Sequencing Uncovers a Hidden Layer of Immune Cell Regulation by SEQadmin2 Started by SEQadmin2, 06-02-2026, 12:03 PM	0 responses 29 views 0 reactions	Last Post by SEQadmin2 06-02-2026, 12:03 PM
DNA Methylation Study Reveals How Epigenetic Changes Pass Between Generations by SEQadmin2 Started by SEQadmin2, 06-02-2026, 11:40 AM	0 responses 23 views 0 reactions	Last Post by SEQadmin2 06-02-2026, 11:40 AM

Working...

Unconfigured Ad