Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    New to RNA seq.. Need help!!!

    Hello All

    I am doing RNA seq using KAPA Stranded RNA-Seq Kit with RiboErase. I am following all the recommendations of the protocol but I seem to lose all my RNA during rRNA depletion step


    Please suggest what to do.
    Tags: None
  • #2
    How do you know? I would suggest to follow the library prep protocol all the way to the end and then assess the library or lack thereof.

    Comment

    Previous template Next

    Latest Articles

    Collapse
    • seqadmin
      Advanced Tools Transforming the Field of Cytogenomics
      by seqadmin


      At the intersection of cytogenetics and genomics lies the exciting field of cytogenomics. It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. By integrating cytogenetic techniques with genomic analysis, researchers can effectively investigate chromosomal abnormalities related to diseases, particularly...
      09-26-2023, 06:26 AM
    • seqadmin
      How RNA-Seq is Transforming Cancer Studies
      by seqadmin



      Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...
      09-07-2023, 11:15 PM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    New Compartment in Mammalian Cells Offers Fresh Perspective on DNA Management by seqadmin
    Started by seqadmin, Yesterday, 07:14 AM
    0 responses
    11 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    Yesterday, 07:14 AM  
    Parasite Challenges Previous Knowledge by Infecting Non-Immune Cells by seqadmin
    Started by seqadmin, 09-29-2023, 09:38 AM
    0 responses
    13 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    09-29-2023, 09:38 AM  
    New CRISPR Tool Holds Potential for Combatting Viruses by seqadmin
    Started by seqadmin, 09-27-2023, 06:57 AM
    0 responses
    14 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    09-27-2023, 06:57 AM  
    Inbreeding May Offer Long-Term Benefits: A Study on Svalbard Reindeer by seqadmin
    Started by seqadmin, 09-26-2023, 07:53 AM
    0 responses
    31 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    09-26-2023, 07:53 AM  
    View All
    Working...
    X