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  • mnkyboy
    Member
    • Mar 2009
    • 87
    #1

    CAGE Libraries on Illumina?

    I was wondering if anyone in here had experience with CAGE (cap analysis gene expression) libraries on the Illumina platform.

    We have someone interested in the lab and have found some info on methods in the literature but I wanted to check in for some hands on experience and tips or potential pitfalls.
    Tags: None
  • mnkyboy
    Member
    • Mar 2009
    • 87
    #2
    Thanks for fixing the title I realized it was pretty bad and then could not edit it (or figure out how to).

    Comment

    • ECO
      --Site Admin--
      • Oct 2007
      • 1360
      #3
      Hahah no problem, it's the least intuitive feature of vBulletin. You have to click "near" the title when viewing the forum thread list (like you're renaming a file via clicking the mouse).

      Comment

      • SEQond
        Member
        • Jul 2010
        • 27
        #4
        I am also interested

        Comment

        • davetang
          Member
          • Jul 2010
          • 11
          #5
          Originally posted by mnkyboy View Post
          I was wondering if anyone in here had experience with CAGE (cap analysis gene expression) libraries on the Illumina platform.

          We have someone interested in the lab and have found some info on methods in the literature but I wanted to check in for some hands on experience and tips or potential pitfalls.
          Have a look at this paper for some perspective on CAGE versus RNA-Seq:

          Comparison of CAGE and RNA-seq transcriptome profiling using clonally amplified and single-molecule next-generation sequencing - PMC
          http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975069/
          CAGE (cap analysis gene expression) and RNA-seq are two major technologies used to identify transcript abundances as well as structures. They measure expression by sequencing from either the 5′ end of capped molecules (CAGE) or tags randomly ...


          Cheers,

          Dave

          Comment

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