CAGE Libraries on Illumina?

[mnkyboy]

I was wondering if anyone in here had experience with CAGE (cap analysis gene expression) libraries on the Illumina platform.

We have someone interested in the lab and have found some info on methods in the literature but I wanted to check in for some hands on experience and tips or potential pitfalls.

[mnkyboy]

Thanks for fixing the title I realized it was pretty bad and then could not edit it (or figure out how to).

[ECO]

Hahah no problem, it's the least intuitive feature of vBulletin. You have to click "near" the title when viewing the forum thread list (like you're renaming a file via clicking the mouse).

[SEQond]

I am also interested

[davetang]

I was wondering if anyone in here had experience with CAGE (cap analysis gene expression) libraries on the Illumina platform.

We have someone interested in the lab and have found some info on methods in the literature but I wanted to check in for some hands on experience and tips or potential pitfalls.

Have a look at this paper for some perspective on CAGE versus RNA-Seq:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975069/

Cheers,

Dave