MapSplice2 gives error if the thread count (-p value) is greater than 2

[mali_s]

Hello! I am using MapSplice2 to generate fusion candidates for Arabidopsis thaliana RNA-Seq data. All the reference fasta files and index files were generated accordingly, as mentioned in the website. (http://www.netlab.uky.edu/p/bioinfo/MapSplice2UserGuide)

I get an error after it evaluates given files/parameters but before starting Mapsplice-multithread when the thread count was -p55. Then I made it run by default which is -p 1. It was working. It also works if the p value is 2. Greater than 2 it does not work

But I want to run it on more number of threads for parallel aligning as it's taking so much time for default setting. Please help me out with this.

[sklab202@localhost ERR1876170]$ python2 /mnt/storage/sklab202/software/MapSplice2/mapsplice.py -1 reads/ERR1876170_1_trim.fastq -2 reads/ERR1876170_2_trim.fastq --fusion -c /mnt/storage/sklab202/genome_files/mapsplice_fasta_files/ -x /mnt/storage/sklab202/geno[sklab202@localhost ERR1876170]$ python2 /mnt/storage/sklab202/software/MapSplice2/mapsplice.py -1 reads/ERR1876170_1_trim.fastq -2 reads/ERR1876170_2_trim.fastq --fusion -c /mnt/storage/sklab202/genome_files/mapsplice_fasta_files/ -x /mnt/storage/sklab202/genome_files/index_files_mapsplice/at_index -p 55 -o masplice_out

-----------------------------------------------
[Thu Jul 22 17:53:58 2021] Beginning Mapsplice run (MapSplice v2.2.1)
[Thu Jul 22 17:53:58 2021] Bin directory: /mnt/storage/sklab202/software/MapSplice2/bin/ 
[Thu Jul 22 17:53:58 2021] Preparing output location masplice_out/
[Thu Jul 22 17:53:58 2021] Checking files or directory: reads/ERR1876170_1_trim.fastq
[Thu Jul 22 17:53:58 2021] Checking files or directory: reads/ERR1876170_2_trim.fastq
[Thu Jul 22 17:53:58 2021] Checking files or directory: /mnt/storage/sklab202/genome_files/mapsplice_fasta_files//
[Thu Jul 22 17:53:58 2021] Checking Bowtie index files
[Thu Jul 22 17:53:58 2021] Inspecting Bowtie index files
[Thu Jul 22 17:53:58 2021] Checking reference sequence length
[Thu Jul 22 17:53:58 2021] Checking consistency of Bowtie index and reference sequence
[Thu Jul 22 17:53:58 2021] Checking read format
-----[Read Format: FASTQ]
-----[Read Type: Pair End]
-----[Total # Reads: 31643736]
-----[Max Read Length: 101]
-----[Min Read Length: 31]
-----[Max Quality Score: 74]
-----[Min Quality Score: 35]
-----[Quality Score Scale: Phred+33]
[Thu Jul 22 17:54:14 2021] Running MapSplice multi-thread
[MapSplice Running Failed]
Error: Running MapSplice multi-thread failed:  -11me_files/index_files_mapsplice/at_index -p 55 -o masplice_out

-----------------------------------------------
[Thu Jul 22 17:53:58 2021] Beginning Mapsplice run (MapSplice v2.2.1)
[Thu Jul 22 17:53:58 2021] Bin directory: /mnt/storage/sklab202/software/MapSplice2/bin/ 
[Thu Jul 22 17:53:58 2021] Preparing output location masplice_out/
[Thu Jul 22 17:53:58 2021] Checking files or directory: reads/ERR1876170_1_trim.fastq
[Thu Jul 22 17:53:58 2021] Checking files or directory: reads/ERR1876170_2_trim.fastq
[Thu Jul 22 17:53:58 2021] Checking files or directory: /mnt/storage/sklab202/genome_files/mapsplice_fasta_files//
[Thu Jul 22 17:53:58 2021] Checking Bowtie index files
[Thu Jul 22 17:53:58 2021] Inspecting Bowtie index files
[Thu Jul 22 17:53:58 2021] Checking reference sequence length
[Thu Jul 22 17:53:58 2021] Checking consistency of Bowtie index and reference sequence
[Thu Jul 22 17:53:58 2021] Checking read format
-----[Read Format: FASTQ]
-----[Read Type: Pair End]
-----[Total # Reads: 31643736]
-----[Max Read Length: 101]
-----[Min Read Length: 31]
-----[Max Quality Score: 74]
-----[Min Quality Score: 35]
-----[Quality Score Scale: Phred+33]
[Thu Jul 22 17:54:14 2021] Running MapSplice multi-thread
[MapSplice Running Failed]
Error: Running MapSplice multi-thread failed:  -11

Also, it does work for another plant species. I get this error only for this species