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10x low fraction of reads in cells
Hi All,
I have run a Mini-Seq to QC my single nuclei (10x v3.1) experiments. In cell ranger, I am seeing "low" fraction of reads in cells aprox 60%. The 10x site says we should be getting like 70%.
Aside from the fraction of reads in cells our samples look good, we can see all expected cell types etc.
Is the low fraction of reads in cells normal
? Any advice on what this means for sample quality?
I have run a Mini-Seq to QC my single nuclei (10x v3.1) experiments. In cell ranger, I am seeing "low" fraction of reads in cells aprox 60%. The 10x site says we should be getting like 70%.
Aside from the fraction of reads in cells our samples look good, we can see all expected cell types etc.
Is the low fraction of reads in cells normal
? Any advice on what this means for sample quality?