RNA-seq data for only 1 gene

[starvingstudent]

Hello all,
Please excuse my naive question!
I'm exploring the differential expression patterns of a particular gene and want to use RNA-seq to corroborate these findings? Is there a clever way to get RNA-seq data for only my gene without having to download all 3gb of data?

Sorry if this question doesn't make sense, I'm just now trying to familiarize myself with analyzing RNA-seq data.

Thanks very much in advance for help with this!

[colindaven]

Not that I know of. 3gb is not too much data really (I presume you mean the reads, and not a reference sequence). You will need multiple datasets of course though.

If your bandwidth at Uni is not too quick home DSL access is usually not too bad for very large datasets.

[pbluescript]

If these are unmapped reads, then it wouldn't be easy.
If these are mapped reads, you can use bamtools with a command like this:
bamtools filter -in [filename] -out [filename] -region chr[#]:[left gene coordinates]..[right gene coordinates]