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Thanks kmcarr for your verification 
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Thanks for your explanation in detail, pbluescript
You are very helpful ^^
Do you familiar with "FPKM"?
Based on isoforms.fpkm_tracking and genes.fpkm_tracking, how can I identify whether which transcript or gene is highly expressed, normal, lower expressed in an organism?
For FPKM value, is it I should consider how raw input read (*.fastq) form or assembly as transcript?
I just quite confusing the exact meaning of "Isoform-level relative abundance in Fragments Per Kilobase of exon model per Million mapped fragments".
Can you just show me with a simple example?
Cufflink assembly result only shown those protein-coding transcript, am I right?
It don't shown any non-protein-coding transcript, snRNA, ncRNA,etc, am I right?
Thanks again for advice.Comment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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