I downloaded the ensemble gtf file from UCSC browser. I find that some transcripts overlap > 90%. e.g. ENSBTAT00000001568 vs ENSBTAT00000046363. Therefore, if reads hit on the first, it will also hit the second. This will affect the gene expression analysis. Can we simply use one of them?
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
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