I downloaded the ensemble gtf file from UCSC browser. I find that some transcripts overlap > 90%. e.g. ENSBTAT00000001568 vs ENSBTAT00000046363. Therefore, if reads hit on the first, it will also hit the second. This will affect the gene expression analysis. Can we simply use one of them?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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