Illumina vs Evrogen DSN

[anar]

Hi

I'm planning to sequence some normalised cDNA libraries for transcriptome discovery. Can someone please explain whether there is any discernable difference between using the Illumina DSN protocol vs the Evrogen DSN protocol? Capturing all polyA
+ mRNAs, in full length, is important.

More generally, I've heard that any kind of enzyme-based normalisation results in drop out of small polyA+ transcripts. Does anyone have experience of this, or the contrary, or know of any techniques to mitigate it?

Thanks!
Anar

[aarodriguezm]

Hi Anar
Normalization with DSN is a very complicated and very sample dependent protocol.
I suggest you to make an Illumina RNA-seq library and sequence it alone in a illumina sequencing lane at least 100 millions reads.
I wish you the best
Alejandro