Dear all,
I've been making Illumina truseq DNA library and I've noticed dimer/trimer peak on bioanalyzer (high sensitivity assay) after PCR amplification. Is there anyone seeing this kind of result before?
![](http://seqanswers.com/forums/attachment.php?attachmentid=817&d=1307499325)
To avoid this problem, I optimised PCR input, which was around 3ng total, and there was no dimer or trimer peak. Do you think it's gonna make less complex library? Is 3ng PCR input enough for final sequencing library?
I've been making Illumina truseq DNA library and I've noticed dimer/trimer peak on bioanalyzer (high sensitivity assay) after PCR amplification. Is there anyone seeing this kind of result before?
To avoid this problem, I optimised PCR input, which was around 3ng total, and there was no dimer or trimer peak. Do you think it's gonna make less complex library? Is 3ng PCR input enough for final sequencing library?
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