Hi there
We are looking at doing a transcriptome experiment with very limited starting material (usually around 10ug total RNA of good quality RIN > 8). We have tried both the Roche magnetic bead kit and Ambion spin column kit for extracting mRNA from total RNA and are unsure as to what the mRNA should look like on the Agilent Bioanalyser. An example of what we have been seeing is attached - is this mRNA or just rubbish?
Cheers
We are looking at doing a transcriptome experiment with very limited starting material (usually around 10ug total RNA of good quality RIN > 8). We have tried both the Roche magnetic bead kit and Ambion spin column kit for extracting mRNA from total RNA and are unsure as to what the mRNA should look like on the Agilent Bioanalyser. An example of what we have been seeing is attached - is this mRNA or just rubbish?
Cheers
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