Does anyone worry about the quantification of expression from total RNA versus ploy A enriched RNA?
I imagine that you would inflate your expression values from total RNA by including reads from immature RNA and I presume that as splicing is a regulated event this might not be even across genes. But, you would not discriminate between immature and mature RNA when doing qPCR from total RNA (the gold standard), so does this matter?
I imagine that you would inflate your expression values from total RNA by including reads from immature RNA and I presume that as splicing is a regulated event this might not be even across genes. But, you would not discriminate between immature and mature RNA when doing qPCR from total RNA (the gold standard), so does this matter?
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