Strange sequencing result of Hi-seq 2000
A normal pair-end 500bp inset library after sequencing by Hi-seq 2000 got a very strange result. The bases content of G and C are equivalent. However, A and T are not equal. Just like the following picture. I have got the feedback that it is library but not sequencing causes this strange phenomenon because the sequencing quality was good. What would cause this during library construction? Many thans!
-
Last edited by angelbo; 08-09-2011, 11:51 PM.
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM