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  • DSN experts?

    I'm looking for traces of microbial RNA in samples with large amounts of human RNA. Does anyone know if I can use DSN treatment to remove not only human rRNA/etc but also some of the less abundant human RNAs? The goal is to reduce the amount of human RNA to a level where (rare?) bacterial sequences can be detected and sequenced.

    Thanks–

  • #2
    Not exactly what you were looking for, but this is a recently published, interesting approach to enriching for non-human RNA:


    "Pathogen detection using short-RNA deep sequencing subtraction and assembly" by Isakov et al.

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    • #3
      I was wondering the same thing - whether you can monkey around with the the DNS Treatment or the incubation time before adding DNA so you remove lower abundant transcripts as well.

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      • #4
        Yes. Illumina has some technical information about this. You should contact them to request it.

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