DSN experts?

[whw]

I'm looking for traces of microbial RNA in samples with large amounts of human RNA. Does anyone know if I can use DSN treatment to remove not only human rRNA/etc but also some of the less abundant human RNAs? The goal is to reduce the amount of human RNA to a level where (rare?) bacterial sequences can be detected and sequenced.

Thanks–

[arolfe]

Not exactly what you were looking for, but this is a recently published, interesting approach to enriching for non-human RNA:

http://bioinformatics.oxfordjournals...7/15/2027.full
"Pathogen detection using short-RNA deep sequencing subtraction and assembly" by Isakov et al.

[schandri]

I was wondering the same thing - whether you can monkey around with the the DNS Treatment or the incubation time before adding DNA so you remove lower abundant transcripts as well.

[ScottC]

Yes. Illumina has some technical information about this. You should contact them to request it.