Dear all,
I've been using qPCR assay with dissociation analysis to check primer dimers or other undesired fragments. I found bird-nesting peak (red plot) at higher temperature and am wondering what this peak means. (c.f. one single peak in the red plot is the one we expected as library)
I've been using qPCR assay with dissociation analysis to check primer dimers or other undesired fragments. I found bird-nesting peak (red plot) at higher temperature and am wondering what this peak means. (c.f. one single peak in the red plot is the one we expected as library)
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