I would like to sequence PCR amplicons with Ilumina. The PCR amplicon are between 200bp to 450bp. These are PCR amplicons from one gene already pooled for one patient.I will index each patient. I have heard that the PCR amplicons might be to small to use with the sample prep kit from Ilumina? Also for sequencing there might be a to big difference between the amplicons to sequence them together. Anyone with experience in ampliconsequencing that can give me some good advice?
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