I guess the title says it all here. I am looking for an RNA-seq protocol which preserves strand-specificity, and came across this paper.
Comprehensive comparative analysis of strand-specific RNA sequencing methods.
Joshua Z Levin,Moran Yassour, Xian Adiconis, Chad Nusbaum, Dawn Anne Thompson, Nir Friedman, Andreas Gnirke & Aviv Regev
The authors have a done a comprehensive comparison study for various methods, and concluded that the dUTP based method (using dUTP for second strand synthesis, followed by UDG digestion) is the best among 7 others.
Since then, Illumina has come up with their Truseq RNA-seq kit.
I was wondering if anyone here has an experience to share about these 2 methods. Which one is better?
Thanks
Comprehensive comparative analysis of strand-specific RNA sequencing methods.
Joshua Z Levin,Moran Yassour, Xian Adiconis, Chad Nusbaum, Dawn Anne Thompson, Nir Friedman, Andreas Gnirke & Aviv Regev
The authors have a done a comprehensive comparison study for various methods, and concluded that the dUTP based method (using dUTP for second strand synthesis, followed by UDG digestion) is the best among 7 others.
Since then, Illumina has come up with their Truseq RNA-seq kit.
I was wondering if anyone here has an experience to share about these 2 methods. Which one is better?
Thanks
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