Hi,
I'm doing library preparation for SOLiD 5500. I have no problem during the process (size selection, dA tail, adaptor ligation etc), until the purification step after amplifying the library. We have no DNA left after purification . I used Agencourt AMPure beads for purification. Is anybody has the same problem and know is/are the cause(s), and know how to solve it? Our group has the same problem before.
Many thanks
I'm doing library preparation for SOLiD 5500. I have no problem during the process (size selection, dA tail, adaptor ligation etc), until the purification step after amplifying the library. We have no DNA left after purification . I used Agencourt AMPure beads for purification. Is anybody has the same problem and know is/are the cause(s), and know how to solve it? Our group has the same problem before.
Many thanks
Comment