Hi,
I'm hoping to collect some information on how you all handle the gel extraction step in the Illumina sample prep protocol. I have had some issues getting a decent yield of DNA out of 2% TAE agarose gels with the Qiagen QiaexII kit. What kits do you find work particularly well? Any tips for sterile gel prep, visualization, and band excision? Does EtBr cause downstream problems? Does anyone use the new(ish) high voltage electrophoresis buffers (Na-borate/Li-borate/Li-acetate) and/or visual stains (crystal violet/methylene blue)?
I'm hoping to collect some information on how you all handle the gel extraction step in the Illumina sample prep protocol. I have had some issues getting a decent yield of DNA out of 2% TAE agarose gels with the Qiagen QiaexII kit. What kits do you find work particularly well? Any tips for sterile gel prep, visualization, and band excision? Does EtBr cause downstream problems? Does anyone use the new(ish) high voltage electrophoresis buffers (Na-borate/Li-borate/Li-acetate) and/or visual stains (crystal violet/methylene blue)?
Comment