Hi Shurjo,
We have a few customer who have tried a 2-25 minute time course of the setting below to shear RNA:
10%dc,5i, 1000cpb.
A customer generated an average shearing size of 400-500b only after a 2 minute treatment using a concentration of less that 100ng/ul. My suggestion would be to use the setting above and carryout an initial test time course of 2-25 minutes depending on the concentration of the RNA sample you will ultimately shear.
Thank you
hamid
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Co ask. Did anyone has the experience for RNA shearing to target size fragment with AFA?Leave a comment:
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Using Covaris S2 for RNA shearing
Does anyone have any experience using the Covaris AFA technology for
RNA fragmentation? While I'm not sure AFA can be used on RNA, if
anyone has tried it I would appreciate any feedback. The Covaris
website hints that AFA may only work for DNA fragmentation: "Double
stranded DNA (unlike RNA) will shear when exposed to the energy of
AFA".
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