Hi, I am working for my Ms thesis on a metagenetic study of the meiofauna and I am going to amplify the 18S region, then sequencing with 454. My question is if I should reshape primers with te A and B adaptors, and the MID tags before amplify my samples.
I read the B adaptor has a biotina than should not be a normal primer. In every works that I read they always amplify 18s with adaptors and mid tags referring as normal primers. Does anyone can help me?
All the best
I read the B adaptor has a biotina than should not be a normal primer. In every works that I read they always amplify 18s with adaptors and mid tags referring as normal primers. Does anyone can help me?
All the best
Comment