High guys I'm writing a discussion on the importance of obtaining high-molecular-weight DNA for fragment library generation before sequencing. I'm finding it hard to find any conclusive literature on this as it seems it is just common knowledge, and i was wondering if anybody had any good links to specific papers?
Also, i've been trying to find if there's a 'washing' step before the fragments get the specific adaptor sequences ligated to them to remove low-quality DNA from the sample, or even to remove fragments slightly too small for the sequencing platform?
Any help on this subject would be greatly appreciated, as it seems I have spent a large portion of the last week searching endlessly on the internet for references to make my discussion at least more than 'you need high-quality DNA cause if you dont have it you can create libraries and then cant sequence'.
Thanks!!!
Also, i've been trying to find if there's a 'washing' step before the fragments get the specific adaptor sequences ligated to them to remove low-quality DNA from the sample, or even to remove fragments slightly too small for the sequencing platform?
Any help on this subject would be greatly appreciated, as it seems I have spent a large portion of the last week searching endlessly on the internet for references to make my discussion at least more than 'you need high-quality DNA cause if you dont have it you can create libraries and then cant sequence'.
Thanks!!!
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