Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • DNA Shearing

    Hi everyone,

    I would like to fragmentate/shear the DNA samples into small fragments prior to the target capture of interested genes. Plan to use sonicator to do it. Do you guys have any idea how should I do it? Can it be done by using the normal probe sonicator in the lab? I think I need to optimize it to get the favourable fragment size.

  • #2
    What make/model sonicator do you have? If you post that info here someone may be able to give you the conditions you need.

    We use the Covaris to fragment genomic DNA into ~200bp fragments. If you can get a consistent temperature in the water bath then it's very reliable in terms of the fragments sizes that are generated.

    Comment


    • #3
      the only sonicator we have here is sonicator utrasonic processor XL and it has never been sonicated DNA before that's why I'm not sure whether I can use it or not.

      Covaris is pretty good based on what I read from the literature and publications just that we don't have it here. Thanks anyway.

      Comment


      • #4
        I'd recommend the Covaris. Just keep an eye on the water levels and make sure the temperature is within guidelines

        Comment


        • #5
          sonicator

          A probe sonicator will work fine. You must match the probe diameter to your sample size. The XL is most likely #XL-2020 from Misonix. Contact me if you want help with probes. [email protected]

          Or you can go to sonicator.com and review the probes that are available. You can match up what you have to the charts to see if you already have the proper equipment.

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Latest Developments in Precision Medicine
            by seqadmin



            Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

            Somatic Genomics
            “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
            05-24-2024, 01:16 PM
          • seqadmin
            Recent Advances in Sequencing Analysis Tools
            by seqadmin


            The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
            05-06-2024, 07:48 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, Yesterday, 01:32 PM
          0 responses
          10 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-24-2024, 07:15 AM
          0 responses
          199 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-23-2024, 10:28 AM
          0 responses
          221 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-23-2024, 07:35 AM
          0 responses
          231 views
          0 likes
          Last Post seqadmin  
          Working...
          X