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Hi All,
I'm not sure if this is the best place to ask, but i'll ask anyway.
So I was extracting DNA from Serum samples and proceeded using the QIAGEN Kit, after spinning the filters with AW1 and AW2 (both wash buffers), I accidentally added SDS to the column/filter instead of Ethanol. I found out about this right after i spinned the columns through with SDS and immediately added more Ethanol (what I was supposed to add) and spinned through it again. I was planning to use the elution to run PCR and DNA analysis, but I was wondering if the addition of SDS would make this DNA useless or interfere with it too much.
ps. I added a small amount of SDS already along with the sample as part of starting material.
Any help would be greatly appreciated.
Thanks!
I'm not sure if this is the best place to ask, but i'll ask anyway.
So I was extracting DNA from Serum samples and proceeded using the QIAGEN Kit, after spinning the filters with AW1 and AW2 (both wash buffers), I accidentally added SDS to the column/filter instead of Ethanol. I found out about this right after i spinned the columns through with SDS and immediately added more Ethanol (what I was supposed to add) and spinned through it again. I was planning to use the elution to run PCR and DNA analysis, but I was wondering if the addition of SDS would make this DNA useless or interfere with it too much.
ps. I added a small amount of SDS already along with the sample as part of starting material.
Any help would be greatly appreciated.
Thanks!
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