Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • QIAGEN MIDI/Minispin Kit

    Hi All,

    I'm not sure if this is the best place to ask, but i'll ask anyway.

    So I was extracting DNA from Serum samples and proceeded using the QIAGEN Kit, after spinning the filters with AW1 and AW2 (both wash buffers), I accidentally added SDS to the column/filter instead of Ethanol. I found out about this right after i spinned the columns through with SDS and immediately added more Ethanol (what I was supposed to add) and spinned through it again. I was planning to use the elution to run PCR and DNA analysis, but I was wondering if the addition of SDS would make this DNA useless or interfere with it too much.

    ps. I added a small amount of SDS already along with the sample as part of starting material.

    Any help would be greatly appreciated.

    Thanks!

  • #2
    What % SDS? My guess is you would have lost a lot of your DNA. Did you save the flow through?

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Best Practices for Single-Cell Sequencing Analysis
      by seqadmin



      While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
      06-06-2024, 07:15 AM
    • seqadmin
      Latest Developments in Precision Medicine
      by seqadmin



      Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

      Somatic Genomics
      “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
      05-24-2024, 01:16 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 07:23 AM
    0 responses
    5 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-17-2024, 06:54 AM
    0 responses
    11 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-14-2024, 07:24 AM
    0 responses
    24 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 06-13-2024, 08:58 AM
    0 responses
    17 views
    0 likes
    Last Post seqadmin  
    Working...
    X