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Hi all,
we are currently working on optimizing our library QC process and wondered if and how we can implement qPCR melting curve analysis to get a rough estimate of the library diversity. Of course we do not seek to get a precise estimate but rather a rough indication if the diversity will be OK or not. Has anyone experience with this? I couldn't find anything online but I would assume that it should be possible to get a an idea of how good or bad a library might be. Thanks in advance.
Cheers,
Zapp
we are currently working on optimizing our library QC process and wondered if and how we can implement qPCR melting curve analysis to get a rough estimate of the library diversity. Of course we do not seek to get a precise estimate but rather a rough indication if the diversity will be OK or not. Has anyone experience with this? I couldn't find anything online but I would assume that it should be possible to get a an idea of how good or bad a library might be. Thanks in advance.
Cheers,
Zapp