Hi,
Before doing my ChIP experiments I optimised the sonication to give me fragments of around 200-300bp. Recently i assessed the quality of chromatin for an experiment and found that the fragmentation was nevertheless incomplete. I did however manage to get a library by size selection at around the 200bp mark. Would such a library be biased in anyway or is it still ok for sequencing?
Thanks for any help.
Nisha
Before doing my ChIP experiments I optimised the sonication to give me fragments of around 200-300bp. Recently i assessed the quality of chromatin for an experiment and found that the fragmentation was nevertheless incomplete. I did however manage to get a library by size selection at around the 200bp mark. Would such a library be biased in anyway or is it still ok for sequencing?
Thanks for any help.
Nisha