Hello,
We are setting up for an illumina MiSeq run using a PCR amplicon-based library (adding the Illumina adaptors using nested PCR). Our results look quite clean via agarose gel (expected size ~310nt), but when our facility ran bioanalyzer we had quite variable results (ranging between 400 and 500nt, depending on the sample). There are seven samples, each with a different barcode, labeled 1-7.
I'm attaching both the gel and the bioanalyzer report - has anyone seen anything like this before?
Thanks,
Michael
We are setting up for an illumina MiSeq run using a PCR amplicon-based library (adding the Illumina adaptors using nested PCR). Our results look quite clean via agarose gel (expected size ~310nt), but when our facility ran bioanalyzer we had quite variable results (ranging between 400 and 500nt, depending on the sample). There are seven samples, each with a different barcode, labeled 1-7.
I'm attaching both the gel and the bioanalyzer report - has anyone seen anything like this before?
Thanks,
Michael
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