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We are developing a workflow for using the Ampliseq Cancer Panel starting with DNA from single cells. We plan to use WGA but wonder if someone could recommend a kit. Genomiphi for example requires 10 ngs. A single cell will have about 6 pgs. Any advice?
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The following paper has a method to purify the Phi29 polymerase away from DNA contamination, which can be a problem when amplifying low concentrations of DNA. I am not sure if there is a commercial product yet.
Blainey, P. C. and S. R. Quake (2011). "Digital MDA for enumeration of total nucleic acid contamination." Nucleic Acids Research 39(4) e19. -
Qiagen has just introduced a product that is a single cell WGA.
Don't have any experience with it but might be a good starting point.Comment
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