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Dear,
I have design two different panels for two different diseases. The dimension of the target regions are the same (about 1.9Mb), in the first I there are 100 genes (only the exons), while in the other one 22 genes (the entire genes, included introns).
I'm using Sure Select enrichment to sequence the samples. I have noticed that, even If the ratio among total generated reads and mapped reads is the same for the two panels (about 95%), the efficiency of the enrichment is lower in panel with included regions. It means that in this last case, I have many reads off targets.
Anyone have any idea for the reason? Thanks a lot
I have design two different panels for two different diseases. The dimension of the target regions are the same (about 1.9Mb), in the first I there are 100 genes (only the exons), while in the other one 22 genes (the entire genes, included introns).
I'm using Sure Select enrichment to sequence the samples. I have noticed that, even If the ratio among total generated reads and mapped reads is the same for the two panels (about 95%), the efficiency of the enrichment is lower in panel with included regions. It means that in this last case, I have many reads off targets.
Anyone have any idea for the reason? Thanks a lot