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Math is not my strong point! I've pooled 12 samples, previously quantified by KAPA quant, based on their size-adjusted concentration. My library peaks average 700 bp. The KAPA quant of the pooled, size-adjusted library is less than I expected (not super low, just a bit lower than I was aiming for). Was the problem just pipetting or should I have pooled based on the non-adjusted concentration? I'm not sure that I haven't adjusted for size twice, and I don't want to overload the MiSeq.
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While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...Today, 07:15 AM -
Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
“We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...05-24-2024, 01:16 PM