Math is not my strong point! I've pooled 12 samples, previously quantified by KAPA quant, based on their size-adjusted concentration. My library peaks average 700 bp. The KAPA quant of the pooled, size-adjusted library is less than I expected (not super low, just a bit lower than I was aiming for). Was the problem just pipetting or should I have pooled based on the non-adjusted concentration? I'm not sure that I haven't adjusted for size twice, and I don't want to overload the MiSeq.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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