We are using the Chemagen system (http://www.chemagen.com/fileadmin/do...n_brochure.pdf) to extract total nucleic acid from blood and stool samples. The process yields a mixture of purified DNA/RNA, but doesn't suggest how to separate the two. Has anyone done this type of separation before? I know Qiagen uses columns in their AllPrep system, but they can't be purchased separately. Most RNAse or DNase protocols I see are designed to remove small amounts of contaminating RNA/DNA, I don't know how they'd do with total nucleic acid?
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If you need both RNA & DNA from the same prep, then split your prep in half. Treat one half with DNase, then run it over a column (qiagen/zymo whatever) to clean up. Base hydrolyze the other half, then EtOH precipitate. -
Thank you very much, that's likely how I'll proceed. I was going to use RNase A, but base hydrolysis is likely more cost effective, especially for a lot of samples.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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