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We are using the Chemagen system (http://www.chemagen.com/fileadmin/do...n_brochure.pdf) to extract total nucleic acid from blood and stool samples. The process yields a mixture of purified DNA/RNA, but doesn't suggest how to separate the two. Has anyone done this type of separation before? I know Qiagen uses columns in their AllPrep system, but they can't be purchased separately. Most RNAse or DNase protocols I see are designed to remove small amounts of contaminating RNA/DNA, I don't know how they'd do with total nucleic acid?
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If you need both RNA & DNA from the same prep, then split your prep in half. Treat one half with DNase, then run it over a column (qiagen/zymo whatever) to clean up. Base hydrolyze the other half, then EtOH precipitate. -
Thank you very much, that's likely how I'll proceed. I was going to use RNase A, but base hydrolysis is likely more cost effective, especially for a lot of samples.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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