Hello Everyone,
We are working on optimizing our TruSeq library preparation to improve our pull-down efficiency in our probes, and I am just curious to if anyone has any insight on this. In the SureSelect protocol (attached) on pg. 44 it states to do a 24 hour incubation on your hybridized samples. In the notes it mentions that you can go up to a 72 hour incubation on these samples (you just have to watch out for evaporation and make sure that that is not too severe).
We want to include our pull-down efficiency on the probes and are curious to if we were to increase this incubation time from 24 hours to 48 hours or even 72 hours if that would increase this? Does anyone have any experience with this? Have you seen any effect from extending the incubation period?
Any insight would be great!
Thanks!
We are working on optimizing our TruSeq library preparation to improve our pull-down efficiency in our probes, and I am just curious to if anyone has any insight on this. In the SureSelect protocol (attached) on pg. 44 it states to do a 24 hour incubation on your hybridized samples. In the notes it mentions that you can go up to a 72 hour incubation on these samples (you just have to watch out for evaporation and make sure that that is not too severe).
We want to include our pull-down efficiency on the probes and are curious to if we were to increase this incubation time from 24 hours to 48 hours or even 72 hours if that would increase this? Does anyone have any experience with this? Have you seen any effect from extending the incubation period?
Any insight would be great!
Thanks!