Hey has anybody tried the new Covaris kit for DNA extraction & purification with the AFA tubes? Any luck?
They use AFA to dissociate the paraffin, instead of xylene, followed by protease K and RnaseA digestion. After this, one can proceed to purification with a column. Covaris claimed that very large DNA fragments could be obtained by this method, peaking at 8kb.
Alternatively, following the digest, one can shear the DNA directly in the same AFA tube to 200-400bp, and then purify. Samples are ready for NGS sample prep.
This seems tempting because we have been using the traditional method to extract DNA with xylene. Gel/bioanalyzer show that DNA is highly fragmented (as everyone else is observing), being a smear ranging from 800 to very short. It would be nice to omit the harsh organic steps to get more intact DNA.
They use AFA to dissociate the paraffin, instead of xylene, followed by protease K and RnaseA digestion. After this, one can proceed to purification with a column. Covaris claimed that very large DNA fragments could be obtained by this method, peaking at 8kb.
Alternatively, following the digest, one can shear the DNA directly in the same AFA tube to 200-400bp, and then purify. Samples are ready for NGS sample prep.
This seems tempting because we have been using the traditional method to extract DNA with xylene. Gel/bioanalyzer show that DNA is highly fragmented (as everyone else is observing), being a smear ranging from 800 to very short. It would be nice to omit the harsh organic steps to get more intact DNA.
Comment