Is anyone aware of the stability and storage recommendations for 5'-biotin-TEG modified oligos?
I'm using a rather expensive biotinylated primer during the PCR enrichment step of a DNA library preparation for later affinity purification using streptavidin beads, but the amplification efficiency has dropped 5-10X from the previous time it was used. Not knowing what else to do with it, I had resuspended the primer originally to 10 uM in water and it's been freeze-thawed a few times. This has never given me the slightest problem with any regular oligo, but I've seen precipitation in free biotin solutions that have been freeze-thawed so it looks like a really bad idea in retrospect...
I'm using a rather expensive biotinylated primer during the PCR enrichment step of a DNA library preparation for later affinity purification using streptavidin beads, but the amplification efficiency has dropped 5-10X from the previous time it was used. Not knowing what else to do with it, I had resuspended the primer originally to 10 uM in water and it's been freeze-thawed a few times. This has never given me the slightest problem with any regular oligo, but I've seen precipitation in free biotin solutions that have been freeze-thawed so it looks like a really bad idea in retrospect...
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