Dear all,
Does anyone have any experience with these kits?
I was able to make one successful build using the Illumina kit and since then has everything failed. I have also tried the similar kit from NEB.
I have used Ambion brain, liver and testes including some internal in-house controls containing known amounts of small RNA species.
How stable are the reagents in the kit?
Should I worry about my samples? I have used the recommend 1 µg input material for both kits. And QC-ed my samples using the BioAnalyzer RNA kit.
Hope that someone has an idea of what could be wrong or how to proceed!
Does anyone have any experience with these kits?
I was able to make one successful build using the Illumina kit and since then has everything failed. I have also tried the similar kit from NEB.
I have used Ambion brain, liver and testes including some internal in-house controls containing known amounts of small RNA species.
How stable are the reagents in the kit?
Should I worry about my samples? I have used the recommend 1 µg input material for both kits. And QC-ed my samples using the BioAnalyzer RNA kit.
Hope that someone has an idea of what could be wrong or how to proceed!
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