Examples of Agilent 2100 Traces (electropherograms)
Here are some examples of Agilent 2100 electropherograms (RNA Nano 6000 assay) for human total RNAs of widely varying quality. RIN numbers reported in the examples range from 1.1 to 10.0. Each total RNA sample was isolated from cultured cells, fresh frozen tissues, or FFPE tissues (indicated in the first column). I would agree with larissa that a three peak trace would be an unusual manifestation of RNA degradation. Having said that, the attached contains many examples RNA qualities from complete degradation to perfectly intact and they can look quite different... Two traces with the same RIN number can look quite different but as the quality and RIN gets higher the consistency improves...
Here are some examples of Agilent 2100 electropherograms (RNA Nano 6000 assay) for human total RNAs of widely varying quality. RIN numbers reported in the examples range from 1.1 to 10.0. Each total RNA sample was isolated from cultured cells, fresh frozen tissues, or FFPE tissues (indicated in the first column). I would agree with larissa that a three peak trace would be an unusual manifestation of RNA degradation. Having said that, the attached contains many examples RNA qualities from complete degradation to perfectly intact and they can look quite different... Two traces with the same RIN number can look quite different but as the quality and RIN gets higher the consistency improves...
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