Hi, we sequence samples using DNA prep with enrichment by illumina. They recommend an average fragment length of 350bp with a uniform electropherogram, usually reaching baseline around 500bp. We follow the protocol closely but have recently been ending up with final library mean fragment length of around 410 bp with an ugly looking electropherogram (attached) with the graph returning to baseline around 700bp. We know this is not a tagmentation issue and our hyb steps have not been changed. We are seeing a large coverage decrease, as well as a high fold 80 base penalty (>2). We suspect it might have something to do with the IPB cleanup, but cannot confirm until we receive a new lot. Can anyone help with this?
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM