Hi all,
I am working on metagenomic sequencing from stool samples collected using Bunny Wipes, followed by DNA extraction with the Qiagen ProFecal kit, and library preparation for downstream NGS on the Element Biosciences AVITI platform.
At the moment, I am consistently obtaining low yields of around 50 ng from the Bunny Wipes extractions, meaning I am only able to load ~20 ng for library preparation. We are experiencing issues with achieving consistent library preparation success at this input level.
Does anyone have any tips or advice for preparing libraries from low-input samples (~20 ng)? I am open to any suitable shotgun library preparation kits, including Elevate Enzymatic Library Prep, Illumina DNA Prep, or other alternatives.
Thank you in advance for any guidance.
I am working on metagenomic sequencing from stool samples collected using Bunny Wipes, followed by DNA extraction with the Qiagen ProFecal kit, and library preparation for downstream NGS on the Element Biosciences AVITI platform.
At the moment, I am consistently obtaining low yields of around 50 ng from the Bunny Wipes extractions, meaning I am only able to load ~20 ng for library preparation. We are experiencing issues with achieving consistent library preparation success at this input level.
Does anyone have any tips or advice for preparing libraries from low-input samples (~20 ng)? I am open to any suitable shotgun library preparation kits, including Elevate Enzymatic Library Prep, Illumina DNA Prep, or other alternatives.
Thank you in advance for any guidance.