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  • nisha barak
    Junior Member
    • Oct 2011
    • 8
    #1

    To sequence or not to sequence?

    Hi,
    I made a library and the bioanalyzer trace revealed three spikes (250, 300, and 350bp) within the normal curve at around 200-400bp I expected to see. I size selected on a gel (after the PCR amplification step) and ran a low molecular weight ladder several lanes away. Somehow, I think the sample was contaminated with ladder. (Attached are the bioanalyzer trace, sample 3 is the affected one. Also attached is the low molecular weight NEB ladder).

    My question is, do you think it would be ok to sequence this library, given that the contaminant shouldn't cluster to the flow cell? Apart from guessing a little on what the concentration excluding the spikes is are there any other issues I should consider?

    Thanks in advance for any help on this!
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