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Hi all,
I am using multiple pippin prep size selections for a ddRADseq project. Today I forgot to add a cover slip over the elution wells before running a cassette. Well I found out why they are important -- afterward there was some visible liquid surrounding the elution wells, likely because they had spilled over. It was not clear whether the liquid from different wells had mixed or not. My question: do I treat these (irreplaceable) samples as contaminated? Has anyone been in this situation before? It will be an expensive mistake to find out via sequencing. Unfortunately I had to size select my entire samples due to low initial [DNA].
Any advice welcome. Thanks!
Paul
I am using multiple pippin prep size selections for a ddRADseq project. Today I forgot to add a cover slip over the elution wells before running a cassette. Well I found out why they are important -- afterward there was some visible liquid surrounding the elution wells, likely because they had spilled over. It was not clear whether the liquid from different wells had mixed or not. My question: do I treat these (irreplaceable) samples as contaminated? Has anyone been in this situation before? It will be an expensive mistake to find out via sequencing. Unfortunately I had to size select my entire samples due to low initial [DNA].
Any advice welcome. Thanks!
Paul