I am preparing the library for single end RNA-seq following the illumina protocol. However, by agilent analysis for library, the two smear bands occur at 200bp and 500 bp. I do not know why the 500 bp band is visible. Who has the same experience? Anyone's idea for this result?
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Originally posted by peromhc View PostWhat stage of the library prep are you at?? Is this the final purified library? What size bands are you selection?
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