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  • skerker
    Member
    • Nov 2009
    • 10

    powerclean Pro

    Anybody have experience in comparing powerclean from MoBio with their new powerclean Pro kit? I'm hoping they produce DNA of the same quality, but I was told that only the regular kit has been tested internally by pacbio.

    I'm using the Pro kit to clean up some gDNA before making a PacBio library.

    If anyone knows whether the regular kit versus the Pro kit is better (or even matters), that would be great. Of course, we can test head-to-head - just curious to know if the answer is already out there...

    Thanks,
    Jeff

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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