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Also, at least for protein quantification you can't use common protein buffers to dissolve your proteins in on the Qubit. You have to use their proprietary buffer and they won't even tell you what's in it.Leave a comment:
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The libraries I made using NanoDrop values were all very good. We bought the Qubit since it's recommended but haven't made libraries using it's values yet.Leave a comment:
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The Qubit is good, the readings are slightly higher than using a bioanalyser but the results are repeatable and much closer to reality than a nanodrop.
The nanodrop is only useful for reading circularised DNA for SOlid long mate pair but it's still not great for this.Leave a comment:
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I heartily recommend Qubit as well.
Has anyone seen this recent piece concerning plastic leaching and DNA quantitation:
http://www.nature.com/news/2010/1004....2010.200.html
The nice thing about the Qubit is it doesn't see anything but dsDNA, unlike the nanodrop.Leave a comment:
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Both Illumina and ABI recommend Qubit over NanoDrop for quantifying DNA.Leave a comment:
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Low throughput DNA quantitation?
I need 200ng and 50ng/ul DNA for my downstream application. I only need to process eight samples a day. What DNA quantitation method should I use?
Is Qubit fluorometer good enough for me???
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The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM -
Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...10-18-2024, 07:11 AM
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