Hi,
Did you end-up sequencing either of these libraries shown in the bioanalyser traces? Did you get good results?
We have libraries with a relatively large peak at around 55bp (we think its primer, and it's as big as the 200bp peak) and wondered if the presence of this would ruin a sequencing run. Does anyone have any insights to offer, please? We have low sample amount so need to avoid another clean-up step if we can, but don't want to sequence rubbish either :-).
Thanks!
Kylie
Did you end-up sequencing either of these libraries shown in the bioanalyser traces? Did you get good results?
We have libraries with a relatively large peak at around 55bp (we think its primer, and it's as big as the 200bp peak) and wondered if the presence of this would ruin a sequencing run. Does anyone have any insights to offer, please? We have low sample amount so need to avoid another clean-up step if we can, but don't want to sequence rubbish either :-).
Thanks!
Kylie
Comment