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  • blanco
    Member
    • Apr 2012
    • 28

    #1

    Small RNA extractions from plasma

    Hi all who have some experience/knowledge in working with RNA from plasma.

    I just did some extractions and was deterred when I ran the RNA Nano on the bioanalyzer and found no trace of RNA.
    I then ran the samples on the 'small RNA' bioanalyzer chip and this time we were able to pick up some RNA.

    Attached below is one of our 'good' results.

    Click image for larger version

Name:	smallRNAfromPlasma.png
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    So, I have a couple of questions:
    Is this trace what one would expect from plasma RNA?
    And, is this sufficient amount of RNA to construct a 'small RNA' library?

    Any kind of help or comments would be appreciated.
    Many thanks,
    blanco

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