Hello,
Has anyone already tried using double stranded cDNA as input for the TruSeq DNA kit, starting at the « Perform End Repair » step? I had a look at the TruSeq RNA and DNA user guides and both kits look similar although I’m not sure adapter concentration and buffer composition are the same. Moreover, any idea of which should be the ideal cDNA input concentration?
Thank you very much for any advice!
Has anyone already tried using double stranded cDNA as input for the TruSeq DNA kit, starting at the « Perform End Repair » step? I had a look at the TruSeq RNA and DNA user guides and both kits look similar although I’m not sure adapter concentration and buffer composition are the same. Moreover, any idea of which should be the ideal cDNA input concentration?
Thank you very much for any advice!
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