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lzembek

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Join Date: Dec 2009

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Shearing methods for 454 8kb libraries

06-11-2010, 06:24 AM

Does anyone routinely use the Covaris to shear circularized 8kb (or 20kb) fragments following circularization in the "454 8kb Paired End Library Preparation?" If so, what kind of library size/read lengths are you getting? Are they in the expected range, ~300-350nt?

Thanks!
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