Originally posted by francicco
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No matter which company you go with, I highly recommend PCR-free, using Covaris to shear your DNA. This eliminates concern of insertion bias and PCR bias, and I am fairly sure PCR-free is generally accepted to show the most even coverage over AT and GC rich regions. If you can get 100-200 ng of DNA from an individual I think PCR-free or very low cycle PCR library prep from an individual is feasible.
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