Is it true that single-stranded DNA libraries are initially BOUND directly to the flow cell as suggested in Illumina's diagrams (see panel b in attached figure). I always assumed they simply hybridized to the primer lawns.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Originally posted by drdna View PostIs it true that single-stranded DNA libraries are initially BOUND directly to the flow cell as suggested in Illumina's diagrams (see panel b in attached figure). I always assumed they simply hybridized to the primer
It seems the description of the initial stages in the this thread aren't quite accurate.
-
-
Originally posted by GenoMax View PostIs this illustration better: http://www.illumina.com/documents/pr...sequencing.pdf
Comment
-
Originally posted by drdna View PostNo, that's the original image that is also inaccurate for a different reason - see original post. It seems that the Illumina folks did not have anyone who understands the technology check the diagrams coming out of their illustration department/provider. I wanted to use an original Illumina illustration in a talk that describes the principle of bridge amplification but the official diagrams will confuse the audience, so I think I'll create my own.
Comment
Latest Articles
Collapse
-
by seqadmin
Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...-
Channel: Articles
09-23-2024, 06:35 AM -
-
by seqadmin
During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.
Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...-
Channel: Articles
09-09-2024, 10:59 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 10-02-2024, 04:51 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
10-02-2024, 04:51 AM
|
||
Started by seqadmin, 10-01-2024, 07:10 AM
|
0 responses
21 views
0 likes
|
Last Post
by seqadmin
10-01-2024, 07:10 AM
|
||
Started by seqadmin, 09-30-2024, 08:33 AM
|
0 responses
25 views
0 likes
|
Last Post
by seqadmin
09-30-2024, 08:33 AM
|
||
Started by seqadmin, 09-26-2024, 12:57 PM
|
0 responses
18 views
0 likes
|
Last Post
by seqadmin
09-26-2024, 12:57 PM
|
Comment